MINK1 Polyclonal antibody

MINK1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13137-1-AP

Print datasheet

Synonyms

B55, GCK family kinase MiNK, hMINK, hMINKbeta, MAP4K6, MEK kinase kinase 6, MEKKK 6, MINK, MINK1, Misshapen like kinase 1, Misshapen/NIK related kinase, YSK2, ZC3



经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lymphoma tissue, human skeletal muscle tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13137-1-AP targets MINK1 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen MINK1 fusion protein Ag3795 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name misshapen-like kinase 1 (zebrafish)
Synonyms B55, GCK family kinase MiNK, hMINK, hMINKbeta, MAP4K6, MEK kinase kinase 6, MEKKK 6, MINK, MINK1, Misshapen like kinase 1, Misshapen/NIK related kinase, YSK2, ZC3
Calculated Molecular Weight 1332 aa, 150 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC034673
Gene Symbol MINK1
Gene ID (NCBI) 50488
RRIDAB_10644151
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N4C8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MINK1 antibody 13137-1-APDownload protocol
IHC protocol for MINK1 antibody 13137-1-APDownload protocol
IP protocol for MINK1 antibody 13137-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

Hum Mol Genet

Lung adenocarcinoma cell-derived exosomes promote M2 macrophage polarization through transmission of miR-3153 to activate the JNK signaling pathway

Authors - Lu Xu
ratWB

Cardiovasc Ther

Effects of Vitamin D Deficiency on the Function of the Cardiac Autonomic Nervous System in Rats.

Authors - Xuemei Luo
mouseIHC

Front Genet

MBD2 mediates Th17 cell differentiation by regulating MINK1 in Th17-dominant asthma

Authors - Zhifeng Chen