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MIOS Polyclonal antibody

MIOS Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20826-1-AP

Print datasheet

Synonyms

MIOS

验证数据展示

  • WB analysis of K-562 using 20826-1-AP

    WB analysis of K-562 using 20826-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 20826-1-AP (MIOS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 20826-1-AP

    IP experiment of HeLa using 20826-1-AP

    IP result of anti-MIOS (IP:20826-1-AP, 4ug; Detection:20826-1-AP 1:300) with HeLa cells lysate 4000ug.

  • IHC staining of mouse skeletal muscle using 20826-1-AP

    IHC staining of mouse skeletal muscle using 20826-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20826-1-AP (MIOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells, rat liver tissue, HeLa cells, PC-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20826-1-AP targets MIOS in WB, IP, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, rat, mouse
Cited Reactivityhuman, pig
Immunogen MIOS fusion protein Ag14802 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name missing oocyte, meiosis regulator, homolog (Drosophila)
Synonyms MIOS
Calculated Molecular Weight 875 aa, 99 kDa
Observed Molecular Weight 99 kDa
GenBank Accession NumberBC005883
Gene Symbol MIOS
Gene ID (NCBI) 54468
RRIDAB_2878747
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NXC5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MIOS antibody 20826-1-APDownload protocol
IHC protocol for MIOS antibody 20826-1-APDownload protocol
IP protocol for MIOS antibody 20826-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Am J Physiol Endocrinol Metab

Intermittent bolus feeding does not enhance protein synthesis, myonuclear accretion, or lean growth more than continuous feeding in a premature piglet model.

Authors - Marko Rudar
View Article
humanWB

JCI Insight

GATOR2-dependent mTORC1 activity is a therapeutic vulnerability in FOXO1 fusion positive rhabdomyosarcoma

Authors - Jacqueline Morales
  • KD Validated
View Article