验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, rat liver tissue, HeLa cells, PC-3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
产品信息
20826-1-AP targets MIOS in WB, IHC, IP, ELISA applications and shows reactivity with human, rat, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, rat, mouse |
| 文献引用反应性 | human, pig |
| 免疫原 | MIOS fusion protein Ag14802 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | missing oocyte, meiosis regulator, homolog (Drosophila) |
| 别名 | MIOS |
| 计算分子量 | 875 aa, 99 kDa |
| 观测分子量 | 99 kDa |
| GenBank蛋白编号 | BC005883 |
| 基因名称 | MIOS |
| Gene ID (NCBI) | 54468 |
| RRID | AB_2878747 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NXC5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MIOS antibody 20826-1-AP | Download protocol |
| IHC protocol for MIOS antibody 20826-1-AP | Download protocol |
| IP protocol for MIOS antibody 20826-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Physiol Endocrinol Metab Intermittent bolus feeding does not enhance protein synthesis, myonuclear accretion, or lean growth more than continuous feeding in a premature piglet model. | ||
JCI Insight GATOR2-dependent mTORC1 activity is a therapeutic vulnerability in FOXO1 fusion positive rhabdomyosarcoma
| ||
Nat Commun Increased translation driven by non-canonical EZH2 creates a synthetic vulnerability in enzalutamide-resistant prostate cancer |
