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验证数据展示

  • IF Staining of U2OS using 83856-4-RR (same clone as 83856-4-PBS)

    IF Staining of U2OS using 83856-4-RR (same clone as 83856-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MIOS antibody (83856-4-RR, Clone: 240832D12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83856-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83856-4-RR

    Affinity and Kinetic Characterization of 83856-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83856-4-RR against Human MIOS were performed. The affinity constant is 0.439 nM.

MIOS Recombinant antibody, PBS Only

MIOS Uni-rAbTM Recombinant Antibody for IF/ICC, Indirect ELISA
Cat No. 83856-4-PBS

产品说明书

CloneNo. 240832D12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, Indirect ELISA

别名

Missing oocyte meiosis regulator homolog, GATOR2 complex protein MIOS, 240832D12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83856-4-PBS targets MIOS in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 MIOS fusion protein Ag15148 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 missing oocyte, meiosis regulator, homolog (Drosophila)
别名 Missing oocyte meiosis regulator homolog, GATOR2 complex protein MIOS, 240832D12
计算分子量 875 aa, 99 kDa
GenBank蛋白编号BC005883
基因名称 MIOS
Gene ID (NCBI) 54468
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NXC5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The mechanistic target of rapamycin complex 1 (mTORC1) controls growth by regulating anabolic and catabolic processes in response to environmental cues, including nutrients. Amino acids signal to mTORC1 through the Rag GTPases, which are regulated by several protein complexes, including GATOR1 and GATOR2. GATOR2, which has five components (WDR24, MIOS, WDR59, SEH1L, SEC13), is required for amino acids to activate mTORC1. PMID: 3583151