• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MIOS Recombinant antibody, PBS Only

MIOS Uni-rAbTM Recombinant Antibody for IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240832D12

Cat no : 83856-4-PBS

Print datasheet

Synonyms

Missing oocyte meiosis regulator homolog, GATOR2 complex protein MIOS, 240832D12

验证数据展示

  • IF Staining of U2OS using 83856-4-RR (same clone as 83856-4-PBS)

    IF Staining of U2OS using 83856-4-RR (same clone as 83856-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MIOS antibody (83856-4-RR, Clone: 240832D12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83856-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83856-4-RR

    Affinity and Kinetic Characterization of 83856-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83856-4-RR against Human MIOS were performed. The affinity constant is 0.439 nM.

产品信息

The immunogen of 83856-4-PBS is MIOS Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen MIOS fusion protein Ag15148 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name missing oocyte, meiosis regulator, homolog (Drosophila)
Synonyms Missing oocyte meiosis regulator homolog, GATOR2 complex protein MIOS, 240832D12
Calculated Molecular Weight 875 aa, 99 kDa
GenBank Accession NumberBC005883
Gene Symbol MIOS
Gene ID (NCBI) 54468
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NXC5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The mechanistic target of rapamycin complex 1 (mTORC1) controls growth by regulating anabolic and catabolic processes in response to environmental cues, including nutrients. Amino acids signal to mTORC1 through the Rag GTPases, which are regulated by several protein complexes, including GATOR1 and GATOR2. GATOR2, which has five components (WDR24, MIOS, WDR59, SEH1L, SEC13), is required for amino acids to activate mTORC1. PMID: 3583151