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MIP-1 alpha/CCL3 Polyclonal antibody

MIP-1 alpha/CCL3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27441-1-AP

Print datasheet

Synonyms

C C motif chemokine 3, CCL 3, CCL3, CCL-3, chemokine (C C motif) ligand 3, G0S19 1, MIP 1 alpha, MIP 1 alpha/CCL3, MIP 1A, MIP-1 alpha, MIP1A, MIP-1α

验证数据展示

  • WB analysis using 27441-1-AP

    WB analysis using 27441-1-AP

    PMA, LPS, and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 27441-1-AP (MIP 1 alpha/CCL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inTHP-1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27441-1-AP targets MIP-1 alpha/CCL3 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen MIP-1 alpha/CCL3 fusion protein Ag26764 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chemokine (C-C motif) ligand 3
Synonyms C C motif chemokine 3, CCL 3, CCL3, CCL-3, chemokine (C C motif) ligand 3, G0S19 1, MIP 1 alpha, MIP 1 alpha/CCL3, MIP 1A, MIP-1 alpha, MIP1A, MIP-1α
Calculated Molecular Weight 92 aa, 10 kDa
Observed Molecular Weight10 kDa
GenBank Accession NumberBC071834
Gene Symbol CCL3/MIP-1 alpha
Gene ID (NCBI) 6348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10147
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chemokine (C-C motif) ligand 3 (CCL3), also known as MIP-1α, belongs to the family of chemokines. CCL3 has been found in the central nervous system and its cognate receptors, CCR1 and CCR5, have been reported to be expressed by astrocytes, microglia and neurons. CCL3 and its receptors, CCR1 and CCR5, also contribute to the development of bone disease in multiple myeloma by supporting tumor growth and regulating osteoclast differentiation. CCL3 is also associated with the regulation of cell growth, angiogenesis, and metastasis of different tumors such as melanoma, renal cell carcinoma, and colorectal cancer. Moreover, CCL3 enhances cell migration and metastasis by up-regulating matrix metalloproteinase-2 (MMP)-2 expression in chondrosarcoma cells.

实验方案

Product Specific Protocols
WB protocol for MIP-1 alpha/CCL3 antibody 27441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols