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MIP-3 Beta Polyclonal antibody, PBS Only

MIP-3 Beta Polyclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 13397-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 13397-1-AP (same clone as 13397-1-PBS)

    WB analysis using 13397-1-AP (same clone as 13397-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 13397-1-AP (MIP-3 Beta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13397-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 13397-1-AP (same clone as 13397-1-PBS)

    IF Staining of A549 using 13397-1-AP (same clone as 13397-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using MIP-3 Beta antibody (13397-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13397-1-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 13397-1-AP (same clone as 13397-1-PBS)

    FC experiment of A549 using 13397-1-AP (same clone as 13397-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human MIP-3 Beta (13397-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 13397-1-PBS in a different storage buffer formulation.

产品信息

13397-1-PBS targets MIP-3 Beta in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen MIP-3 Beta fusion protein Ag4200 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chemokine (C-C motif) ligand 19
Synonyms
Calculated Molecular Weight 98 aa, 12 kDa
GenBank Accession NumberBC027968
Gene Symbol CCL19/MIP-3 beta
Gene ID (NCBI) 6363
RRIDAB_2071529
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99731
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCL19, also known as MIP-3β, is one of several CC cytokine genes clustered on the p-arm of chromosome 9. CCL19 may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7.