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MIPOL1 Polyclonal antibody

MIPOL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14158-1-AP

Print datasheet

Synonyms

MIPOL1, mirror image polydactyly 1

验证数据展示

  • WB analysis of HepG2 using 14158-1-AP

    WB analysis of HepG2 using 14158-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14158-1-AP (MIPOL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 14158-1-AP

    IHC staining of human liver cancer using 14158-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14158-1-AP (MIPOL1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver cancer using 14158-1-AP

    IHC staining of human liver cancer using 14158-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14158-1-AP (MIPOL1 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inHepG2 cells, human liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14158-1-AP targets MIPOL1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen MIPOL1 fusion protein Ag5340 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mirror-image polydactyly 1
Synonyms MIPOL1, mirror image polydactyly 1
Calculated Molecular Weight 442 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC035870
Gene Symbol MIPOL1
Gene ID (NCBI) 145282
RRIDAB_2141930
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TD10
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MIPOL1 antibody 14158-1-APDownload protocol
IHC protocol for MIPOL1 antibody 14158-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols