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MIS12 Polyclonal antibody

MIS12 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 19820-1-AP

Print datasheet

Synonyms

2510025F08Rik, hMis12, KNTC2AP, MIS12, MTW1, Protein MIS12 homolog

验证数据展示

  • WB analysis using 19820-1-AP

    WB analysis using 19820-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19820-1-AP (MIS12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19820-1-AP targets MIS12 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MIS12 fusion protein Ag13870 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name MIS12, MIND kinetochore complex component, homolog (yeast)
Synonyms 2510025F08Rik, hMis12, KNTC2AP, MIS12, MTW1, Protein MIS12 homolog
Calculated Molecular Weight 205 aa, 24 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC000229
Gene Symbol MIS12
Gene ID (NCBI) 79003
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H081
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The MIS12 complex, composed of the Mis12, Pmf1, Nsl1 and Dsn1 subunits, is a highly conserved kinetochore protein that interacts with the Ndc80 and Knl1 complexes, forming the KMN network (PMID: 32341029). MIS12 is a part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. MIS12 is essential for proper kinetochore microtubule attachments (PubMed:23891108)

实验方案

Product Specific Protocols
WB protocol for MIS12 antibody 19820-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols