• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MIST1 Polyclonal antibody

MIST1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29853-1-AP

Print datasheet

Synonyms

BHLHA15, BHLHB8, MIST 1, MIST1

验证数据展示

  • WB analysis using 29853-1-AP

    WB analysis using 29853-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29853-1-AP (MIST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse pancreas tissue, rat pancreas tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29853-1-AP targets MIST1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen MIST1 fusion protein Ag32096 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name basic helix-loop-helix family, member a15
Synonyms BHLHA15, BHLHB8, MIST 1, MIST1
Calculated Molecular Weight21 kDa
Observed Molecular Weight20-30 kDa
GenBank Accession NumberNM_177455
Gene Symbol MIST1
Gene ID (NCBI) 168620
RRIDAB_2935483
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7RTS1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Muscle intestine stomach expression 1 (MIST1), also named BHLHA15, is a secretory cell transcription factor that belongs to the basic helix-loop-helix superfamily. MIST1 is mainly expressed in serous secreted cells and essential for the development and maturation of secretory cells. Secretory cells lacking MIST1 exhibit structural and functional defects, including alterations in cytoskeletal organization and cell polarity and improper cell-cell junction. In healthy pancreata, MIST1 facilitates cell-to-cell communication, promotes acinar cell polarity, controls Ca2+ flux, promotes unfolded protein response (UPR) homeostasis, aids in vesicle assembly, and is required for regulated exocytosis. MIST1 was downregulated in pancreatic cancer tissues compared with normal or adjacent pancreatic tissues and lower MIST1 expression in pancreatic cancer cell lines than normal acinar cells (PMID:29859299).

实验方案

Product Specific Protocols
WB protocol for MIST1 antibody 29853-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols