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  • Featured Product
  • KD/KO Validated

Muskelin Polyclonal antibody

Muskelin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 14735-1-AP

Print datasheet

Synonyms

MKLN1, Muskelin, TWA2

验证数据展示

  • WB analysis of Y79 using 14735-1-AP

    WB analysis of Y79 using 14735-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 14735-1-AP

    IHC staining of human gliomas using 14735-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 14735-1-AP (Muskelin antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inY79 cells, Jurkat cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14735-1-AP targets Muskelin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Muskelin fusion protein Ag6147 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name muskelin 1, intracellular mediator containing kelch motifs
Synonyms MKLN1, Muskelin, TWA2
Calculated Molecular Weight 735 aa, 85 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC067825
Gene Symbol Muskelin
Gene ID (NCBI) 4289
RRIDAB_2142504
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UL63
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Muskelin antibody 14735-1-APDownload protocol
IHC protocol for Muskelin antibody 14735-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IP,IF

Neuron

Muskelin Coordinates PrPC Lysosome versus Exosome Targeting and Impacts Prion Disease Progression.

Authors - Frank F Heisler
  • KO Validated
View Article
humanWB

Sci Rep

Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin.

Authors - Shimaa H A Soliman
View Article
humanWB,IP

Mol Cell

mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway

Authors - Sang Ah Yi
  • KO Validated
View Article