验证数据展示
经过测试的应用
| Positive WB detected in | mouse spleen tissue, MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
产品信息
17354-1-AP targets MKNK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | MKNK2 fusion protein Ag10546 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | MAP kinase interacting serine/threonine kinase 2 |
| 别名 | MNK2, MAPK signal-integrating kinase 2, MAP kinase-interacting serine/threonine-protein kinase 2, MAP kinase signal-integrating kinase 2, GPRK7 |
| 计算分子量 | 465 aa, 52 kDa |
| 观测分子量 | 47-52 kDa |
| GenBank蛋白编号 | BC018345 |
| 基因名称 | MKNK2 |
| Gene ID (NCBI) | 2872 |
| RRID | AB_2878395 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HBH9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MKNK2(MAP kinase-interacting serine/threonine-protein kinase 2) is also named as GPRK7, MNK2 and belongs to the CAMK Ser/Thr protein kinase family. It has a conserved C-terminal Erk-interacting domain, a catalytic domain with homology to the calcium/calmodulin-dependent family of kinases, and putative MAP kinase phosphorylation sites located within the T loop of the kinase domain(PMID:9155017). The breast cancer cells express either an uncharacterized form of MNK2 or a cross-reacting MNK2-like protein with the band of 72-75 kDa by western blot(PMID:17130135). MKNK2 has 2 isoforms with the molecular mass of 52 kDa and 47 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MKNK2 antibody 17354-1-AP | Download protocol |
| IHC protocol for MKNK2 antibody 17354-1-AP | Download protocol |
| IF protocol for MKNK2 antibody 17354-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Clin Sci (Lond) Down-regulation of the insulin signaling pathway by SHC may correlate with congenital heart disease in Chinese populations. | ||
Oncotarget Impact of eIF4E phosphorylation at Ser209 via MNK2a on tumour recurrence after curative surgery in localized clear cell renal cell carcinoma. | ||
Inflammation High Expression of miR-6785-5p in the Serum Exosomes of Psoriasis Patients Alleviates Psoriasis-Like Skin Damage by Interfering with the MNK2/p-eIF4E Axis in Keratinocytes
| ||
J Agric Food Chem Non-Sugar Sweetener Rubusoside Alleviates Lipid Metabolism Disorder In Vivo and In Vitro by Targeting PPARγ/α, Lgals3, and Mknk2 |
