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- KD/KO Validated
CoraLite® Plus 488-conjugated MKP-2 Monoclonal antibody
MKP-2 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
human, pig, rat, mouse
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1G1C12
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66349 targets MKP-2 in IF applications and shows reactivity with human, pig, rat, mouse samples.
Tested Applications | IF Application Description |
Tested Reactivity | human, pig, rat, mouse |
Immunogen | MKP-2 fusion protein Ag1206 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | dual specificity phosphatase 4 |
Synonyms | dual specificity phosphatase 4, DUSP4, HVH2, MAP kinase phosphatase 2, MKP 2, MKP2, MKP-2, TYP, VH2 |
Calculated Molecular Weight | 43 kDa |
Observed Molecular Weight | 43 kDa, 33 kDa |
GenBank Accession Number | BC014565 |
Gene Symbol | DUSP4 |
Gene ID (NCBI) | 1846 |
RRID | AB_2883315 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q13115 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DUSP4, also named as MKP-2, is a member of the mitogen-activated protein (MAP) kinase phosphatase family which has been suggested to play an important role in the feedback control of MAP kinase-mediated gene expression. DUSP4 preferentially inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. DUSP4 encodes two distinct isoforms: MKP-2-L(43 kDa) and MKP-2-S (33 kDa).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 MKP-2 antibody CL488-66349 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |