验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, mouse brain tissue, mouse thymus tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12773-1-AP targets MKRN1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | MKRN1 fusion protein Ag3465 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | makorin ring finger protein 1 |
| 别名 | RNF61, RING-type E3 ubiquitin transferase makorin-1, RING finger protein 61, makorin ring finger protein 1, EC:2.3.2.27 |
| 计算分子量 | 482 aa, 53 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC025955 |
| 基因名称 | MKRN1 |
| Gene ID (NCBI) | 23608 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UHC7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MKRN1 antibody 12773-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |