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MLF1 Polyclonal antibody

MLF1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat No : 13100-1-AP

Print datasheet

Synonyms

MLF1, myeloid leukemia factor 1

验证数据展示

  • WB analysis of mouse heart using 13100-1-AP

    WB analysis of mouse heart using 13100-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 13100-1-AP (MLF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 13100-1-AP

    IHC staining of human colon cancer using 13100-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13100-1-AP (MLF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MLF1 antibody included
  • IHC staining of human colon cancer using 13100-1-AP

    IHC staining of human colon cancer using 13100-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13100-1-AP (MLF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MLF1 antibody included
  • IP experiment of mouse heart using 13100-1-AP

    IP experiment of mouse heart using 13100-1-AP

    IP result of anti-MLF1 (IP:13100-1-AP, 4ug; Detection:13100-1-AP 1:500) with mouse heart tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse heart tissue, human brain tissue, mouse testis tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MLF1 Ready-To-Use IHC Kit. MLF1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13100-1-AP targets MLF1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen MLF1 fusion protein Ag3727 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myeloid leukemia factor 1
Synonyms MLF1, myeloid leukemia factor 1
Calculated Molecular Weight 268 aa, 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC007045
Gene Symbol MLF1
Gene ID (NCBI) 4291
RRIDAB_2147670
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP58340
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MLF1 antibody 13100-1-APDownload protocol
IHC protocol for MLF1 antibody 13100-1-APDownload protocol
IP protocol for MLF1 antibody 13100-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

CRL4DCAF8 and USP11 oppositely regulate the stability of myeloid leukemia factors (MLFs).

Authors - Daoyuan Huang
View Article