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验证数据展示

  • WB analysis of mouse heart using 13100-1-AP

    WB analysis of mouse heart using 13100-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 13100-1-AP (MLF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 13100-1-AP

    IHC staining of human colon cancer using 13100-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13100-1-AP (MLF1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MLF1 antibody included
  • IHC staining of human colon cancer using 13100-1-AP

    IHC staining of human colon cancer using 13100-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13100-1-AP (MLF1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MLF1 antibody included
  • IP experiment of mouse heart using 13100-1-AP

    IP experiment of mouse heart using 13100-1-AP

    IP result of anti-MLF1 (IP:13100-1-AP, 4ug; Detection:13100-1-AP 1:500) with mouse heart tissue lysate 4000ug.

MLF1 Polyclonal antibody

MLF1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 13100-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, ELISA

别名

myeloid leukemia factor 1, Myelodysplasia-myeloid leukemia factor 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, human brain tissue, mouse testis tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MLF1 Ready-To-Use IHC Kit. MLF1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13100-1-AP targets MLF1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 MLF1 fusion protein Ag3727 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myeloid leukemia factor 1
别名 myeloid leukemia factor 1, Myelodysplasia-myeloid leukemia factor 1
计算分子量 268 aa, 31 kDa
观测分子量 31 kDa
GenBank蛋白编号BC007045
基因名称 MLF1
Gene ID (NCBI) 4291
RRIDAB_2147670
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP58340
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MLF1 antibody 13100-1-APDownload protocol
IHC protocol for MLF1 antibody 13100-1-APDownload protocol
IP protocol for MLF1 antibody 13100-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

CRL4DCAF8 and USP11 oppositely regulate the stability of myeloid leukemia factors (MLFs).

Authors - Daoyuan Huang
View Article