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验证数据展示

  • WB analysis of human brain using 11835-1-AP

    WB analysis of human brain using 11835-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11835-1-AP (MLF2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 11835-1-AP

    IHC staining of human gliomas using 11835-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11835-1-AP (MLF2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 11835-1-AP

    IHC staining of human gliomas using 11835-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11835-1-AP (MLF2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 11835-1-AP

    IF Staining of HeLa using 11835-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11835-1-AP (MLF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MLF2 Polyclonal antibody

MLF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11835-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

NTN4, Myeloid leukemia factor 2, Myelodysplasia-myeloid leukemia factor 2, MLF 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11835-1-AP targets MLF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原 MLF2 fusion protein Ag2270 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myeloid leukemia factor 2
别名 NTN4, Myeloid leukemia factor 2, Myelodysplasia-myeloid leukemia factor 2, MLF 2
计算分子量 248 aa, 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC000898
基因名称 MLF2
Gene ID (NCBI) 8079
RRIDAB_2235413
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15773
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MLF2 antibody 11835-1-APDownload protocol
IHC protocol for MLF2 antibody 11835-1-APDownload protocol
IF protocol for MLF2 antibody 11835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Oncol

Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis

Authors - Tianwei Chen
View Article
humanIF

Adv Sci (Weinh)

MLF2 Negatively Regulates P53 and Promotes Colorectal Carcinogenesis

Authors - Debao Fang
View Article
humanIF

J Am Soc Nephrol

Integrated Single-Cell Transcriptomic Atlas of Human Kidney Endothelial Cells

Authors - Ka Zhang
View Article