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验证数据展示

  • WB analysis using 31396-1-AP

    WB analysis using 31396-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31396-1-AP (MLL4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 31396-1-AP

    IF Staining of U2OS using 31396-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MLL4 antibody (31396-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

MLL4 Polyclonal antibody

MLL4 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31396-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

MLL1B, KMT2B, KIAA0304, HRX2, DYT28

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31396-1-AP targets MLL4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35385

Product name: Recombinant human MLL4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 800-982 aa of BC009337

Sequence: KVQLFKIDQQQQQKVAASMPLSPGGQMEEVAGAVKQISDRGPVRSEDESVEAKRERPSGPESPVQGPRIKHVCRHAAVALGQARAMVPEDVPRLSALPLRDRQDLATEDTSSASETESVPSRSRRGKVEAAGPGGESEPTGSGGTLAHTPRRSLPSHHGKKMRMARCGHCRGCLRVQDCGSCV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myeloid/lymphoid or mixed-lineage leukemia 4
别名 MLL1B, KMT2B, KIAA0304, HRX2, DYT28
观测分子量180kDa;320kDa
GenBank蛋白编号BC009337
基因名称 MLL4
Gene ID (NCBI) 9757
RRIDAB_3669963
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9UMN6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MLL4, also known as KMT2B, which is an essential subunit of the H3K4 methylation complexes, regulates cell type-specific transcriptional programs through enhancer activation. Taspase 1 mediates cleavage of the MLL protein, generating 320 kDa N-terminal (MLL-N) and 180 kDa C-terminal (MLL-C) fragments (PMID: 24213472).

实验方案

Product Specific Protocols
IF protocol for MLL4 antibody 31396-1-APDownload protocol
WB protocol for MLL4 antibody 31396-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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