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验证数据展示

  • WB analysis using 31029-1-AP

    WB analysis using 31029-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31029-1-AP (MLLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 31029-1-AP

    IF Staining of HeLa using 31029-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MLLT1 antibody (31029-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

MLLT1 Polyclonal antibody

MLLT1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31029-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

ENL, LTG19, MLLT1, Protein ENL, YEATS1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMDA-MB-453 cells, HeLa cells, Jurkat cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31029-1-AP targets MLLT1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen MLLT1 fusion protein Ag34210 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 1
Synonyms ENL, LTG19, MLLT1, Protein ENL, YEATS1
Calculated Molecular Weight62 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC148806
Gene Symbol MLLT1
Gene ID (NCBI) 4298
RRIDAB_3669821
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ03111
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MLLT1 antibody 31029-1-APDownload protocol
IF protocol for MLLT1 antibody 31029-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols