验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells |
| Positive IP detected in | A375 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, MCF-7 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10338-1-AP targets MLPH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | MLPH fusion protein Ag0316 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | melanophilin |
| Synonyms | SlaC2-a, SLAC2A, SLAC2 A, Melanophilin, Exophilin-3 |
| Calculated Molecular Weight | 66 kDa |
| Observed Molecular Weight | 66 kDa, 80 kDa |
| GenBank Accession Number | BC001653 |
| Gene Symbol | MLPH |
| Gene ID (NCBI) | 79083 |
| RRID | AB_2146104 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BV36 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MLPH antibody 10338-1-AP | Download protocol |
| IHC protocol for MLPH antibody 10338-1-AP | Download protocol |
| IF protocol for MLPH antibody 10338-1-AP | Download protocol |
| IP protocol for MLPH antibody 10338-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Allergy Clin Immunol Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding. | ||
Theranostics A novel function of Prohibitin on melanosome transport in melanocytes.
| ||
Oncogene O-GlcNAcylation of melanophilin enhances radiation resistance in glioblastoma via suppressing TRIM21 mediated ubiquitination | ||
Elife Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis | ||
Part Fibre Toxicol Coal dust exposure triggers heterogeneity of transcriptional profiles in mouse pneumoconiosis and Vitamin D remedies. | ||
J Invest Dermatol Knockdown of myosin Va isoforms by RNAi as a tool to block melanosome transport in primary human melanocytes. |
