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验证数据展示

  • WB analysis using 30618-1-AP

    WB analysis using 30618-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30618-1-AP (MLPH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 30618-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30618-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30618-1-AP (MLPH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 30618-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30618-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30618-1-AP (MLPH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MLPH Polyclonal antibody

MLPH Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30618-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

SlaC2-a, SLAC2A, SLAC2 A, Melanophilin, Exophilin-3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HT-29 cells, LNCaP cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30618-1-AP targets MLPH in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 MLPH fusion protein Ag32931 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 melanophilin
别名 SlaC2-a, SLAC2A, SLAC2 A, Melanophilin, Exophilin-3
计算分子量 66 kDa
观测分子量66 kDa, 80 kDa
GenBank蛋白编号BC001653
基因名称 MLPH
Gene ID (NCBI) 79083
RRIDAB_3669739
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BV36
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.

实验方案

Product Specific Protocols
WB protocol for MLPH antibody 30618-1-APDownload protocol
IHC protocol for MLPH antibody 30618-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols