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验证数据展示

  • WB analysis using 30618-1-AP

    WB analysis using 30618-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30618-1-AP (MLPH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 30618-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30618-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30618-1-AP (MLPH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 30618-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30618-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30618-1-AP (MLPH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MLPH Polyclonal antibody

MLPH Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30618-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

SlaC2-a, SLAC2A, SLAC2 A, Melanophilin, Exophilin-3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HT-29 cells, LNCaP cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30618-1-AP targets MLPH in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen MLPH fusion protein Ag32931 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name melanophilin
Synonyms SlaC2-a, SLAC2A, SLAC2 A, Melanophilin, Exophilin-3
Calculated Molecular Weight 66 kDa
Observed Molecular Weight66 kDa, 80 kDa
GenBank Accession NumberBC001653
Gene Symbol MLPH
Gene ID (NCBI) 79083
RRIDAB_3669739
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BV36
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.

实验方案

Product Specific Protocols
WB protocol for MLPH antibody 30618-1-APDownload protocol
IHC protocol for MLPH antibody 30618-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols