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  • Featured Product
  • KD/KO Validated

MLXIP Polyclonal antibody

MLXIP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 13614-1-AP

Print datasheet

Synonyms

MLX-interacting protein, MLX interacting protein, MIR, KIAA0867, Class E basic helix-loop-helix protein 36

验证数据展示

  • IHC staining of human skeletal muscle using 13614-1-AP

    IHC staining of human skeletal muscle using 13614-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 13614-1-AP (MLXIP antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with MLXIP antibody included
  • IHC staining of human skeletal muscle using 13614-1-AP

    IHC staining of human skeletal muscle using 13614-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 13614-1-AP (MLXIP antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with MLXIP antibody included
  • IF Staining of HepG2 using 13614-1-AP

    IF Staining of HepG2 using 13614-1-AP

    Immunofluorescent analysis of HepG2 cells, using MLXIP antibody 13614-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • WB analysis of K-562 using 13614-1-AP

    WB analysis of K-562 using 13614-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13614-1-AP (MLXIP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 13614-1-AP

    IP experiment of K-562 using 13614-1-AP

    IP result of anti-MLXIP (IP:13614-1-AP, 4ug; Detection:13614-1-AP 1:300) with K-562 cells lysate 3200ug.

  • IP experiment of K-562 using 13614-1-AP

    IP experiment of K-562 using 13614-1-AP

    IP result of anti-MLXIP (IP:13614-1-AP, 4ug; Detection:13614-1-AP 1:300) with K-562 cells lysate 3200ug.

经过测试的应用

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MLXIP Ready-To-Use IHC Kit. MLXIP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13614-1-AP targets MLXIP in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen MLXIP fusion protein Ag4519 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name MLX interacting protein
Synonyms MLX-interacting protein, MLX interacting protein, MIR, KIAA0867, Class E basic helix-loop-helix protein 36
Calculated Molecular Weight 919 aa, 100 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC039704
Gene Symbol MLXIP
Gene ID (NCBI) 22877
RRIDAB_2282153
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HAP2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mlx-interacting protein(MLXIP), after forming heterodimers with MLX, can bind to and then activate transcription from CACGTG E boxes. The N terminus of MLXIP acts as a CRM1-dependent nuclear export signal and contribute to the cytoplasmic localization of the complex, and as a binding site for 13 protein. the C terminus contains a cytoplasmic localization signal and mediates protein-protein interaction of MONDOA and MLX. The complex play a role in transcriptional activation of glycolytic target genes and glucose-responsive gene regulation. This antibody could recognize isoform1(~110kd) and isoform3(~69kd) of MLXIP. A prominent and specific MLXIP of approximately 130 kDa was detected. It demonstrates that MLXIP associated specifically with Mlx in the cytoplasm (PMID: 11073985).

实验方案

Product Specific Protocols
WB protocol for MLXIP antibody 13614-1-APDownload protocol
IHC protocol for MLXIP antibody 13614-1-APDownload protocol
IF protocol for MLXIP antibody 13614-1-APDownload protocol
IP protocol for MLXIP antibody 13614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Cell

Deregulated Myc requires MondoA/Mlx for metabolic reprogramming and tumorigenesis.

Authors - Patrick A Carroll
View Article
mousechIP

Nat Neurosci

PMID: 31636448

Authors - Hei-Man Chow
View Article
mouseWB,FC,ChIP

Gastroenterology

MondoA-Thioredoxin-Interacting Protein Axis Maintains Regulatory T-Cell Identity and Function in Colorectal Cancer Microenvironment.

Authors - Ying Lu
View Article
humanChIP

Proc Natl Acad Sci U S A

Metabolic reprogramming in triple-negative breast cancer through Myc suppression of TXNIP.

Authors - Liangliang Shen
View Article
humanWB

Diabetes

MondoA is an Essential Glucose Responsive Transcription Factor in Human Pancreatic Beta Cells.

Authors - Paul Richards
View Article
humanWB

PLoS Pathog

Latent KSHV Infected Endothelial Cells Are Glutamine Addicted and Require Glutaminolysis for Survival.

Authors - Erica L Sanchez
View Article