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验证数据展示

  • IHC staining of human liver using 21462-1-AP

    IHC staining of human liver using 21462-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21462-1-AP (MMACHC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 21462-1-AP

    IHC staining of human liver using 21462-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21462-1-AP (MMACHC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Raji using 21462-1-AP

    WB analysis of Raji using 21462-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 21462-1-AP (MMACHC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

MMACHC Polyclonal antibody

MMACHC Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21462-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

cblC, MMACHC, RP11 291L19.3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, HeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21462-1-AP targets MMACHC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MMACHC fusion protein Ag15001 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria
别名 cblC, MMACHC, RP11 291L19.3
计算分子量 282 aa, 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC006122
基因名称 MMACHC
Gene ID (NCBI) 25974
RRIDAB_10732959
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y4U1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMACHC, also named as CblC, is located on chromosome 1 and comprises four coding exons. Human MMACHC catalyzes the reductive decyanation of cyanocobalamin and may serve as a trafficking chaperone for intracellular cobalamin. It also catalyzes the glutathione-dependent reductive demethylation of methylcobalamin, and, with much lower efficiency, the glutathione-dependent reductive demethylation of adenosylcobalamin. NMACHC binds cyanocobalamin, adenosylcobalamin, methylcobalamin and other, related vitamin B12 derivatives.

实验方案

Product Specific Protocols
WB protocol for MMACHC antibody 21462-1-APDownload protocol
IHC protocol for MMACHC antibody 21462-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols