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MMACHC Monoclonal antibody

MMACHC Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

4G10A11

Cat no : 66609-1-Ig

Print datasheet

Synonyms

Cyanocobalamin reductase / alkylcobalamin dealkylase, Cyanocobalamin reductase, cyanide-eliminating, CblC, Alkylcobalamin:glutathione S-alkyltransferase

验证数据展示

  • WB analysis of HepG2 using 66609-1-Ig

    WB analysis of HepG2 using 66609-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66609-1-Ig (MMACHC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66609-1-Ig

    IF Staining of HepG2 using 66609-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MMACHC antibody (66609-1-Ig, Clone: 4G10A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HeLa using 66609-1-Ig

    FC experiment of HeLa using 66609-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug Anti-Human MMACHC (66609-1-Ig, Clone:4G10A11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells, HEK-293 cells, HeLa cells, Raji cells, RAW 264.7 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

66609-1-Ig targets MMACHC in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Immunogen MMACHC fusion protein Ag15001 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria
Synonyms Cyanocobalamin reductase / alkylcobalamin dealkylase, Cyanocobalamin reductase, cyanide-eliminating, CblC, Alkylcobalamin:glutathione S-alkyltransferase
Calculated Molecular Weight 282 aa, 32 kDa
GenBank Accession NumberBC006122
Gene Symbol MMACHC
Gene ID (NCBI) 25974
RRIDAB_2881969
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y4U1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMACHC, also named as CblC, is located on chromosome 1 and comprises four coding exons. Human MMACHC catalyzes the reductive decyanation of cyanocobalamin and may serve as a trafficking chaperone for intracellular cobalamin. It also catalyzes the glutathione-dependent reductive demethylation of methylcobalamin, and, with much lower efficiency, the glutathione-dependent reductive demethylation of adenosylcobalamin. NMACHC binds cyanocobalamin, adenosylcobalamin, methylcobalamin and other, related vitamin B12 derivatives.

实验方案

Product Specific Protocols
WB protocol for MMACHC antibody 66609-1-IgDownload protocol
IF protocol for MMACHC antibody 66609-1-IgDownload protocol
FC protocol for MMACHC antibody 66609-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Lett

CYLD deficiency enhances metabolic reprogramming and tumor progression in nasopharyngeal carcinoma via PFKFB3.

Authors - Lingzhi Wang
View Article
ratWB

Mol Neurobiol

Surfeit Locus Protein 4 as a Novel Target for Therapeutic Intervention in Cerebral Ischemia-Reperfusion Injury

Authors - Wenjie Hu
View Article