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  • Featured Product
  • KD/KO Validated

Neprilysin/CD10 Monoclonal antibody

Neprilysin/CD10 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4B9D9

Cat no : 60034-3-Ig

Print datasheet

Synonyms

MME, MME,CD10, Enkephalinase, EC:3.4.24.11, CD10

验证数据展示

  • WB analysis of HEK-293 using 60034-3-Ig

    WB analysis of HEK-293 using 60034-3-Ig

    WB result of Neprilysin/CD10 antibody (60034-3-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Neprilysin/CD10 transfected HEK-293 cells.

  • IHC staining of human kidney using 60034-3-Ig

    IHC staining of human kidney using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD10 antibody included
  • IHC staining of human kidney using 60034-3-Ig

    IHC staining of human kidney using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD10 antibody included
  • IHC staining of human liver using 60034-3-Ig

    IHC staining of human liver using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD10 antibody included
  • IHC staining of human liver using 60034-3-Ig

    IHC staining of human liver using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD10 antibody included
  • WB analysis of pig kidney using 60034-3-Ig

    WB analysis of pig kidney using 60034-3-Ig

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inpig kidney tissue, mouse kidney tissue, HEK-293 cells
Positive IHC detected inhuman kidney tissue, human liver tissue, human renal cell carcinoma tissue, human stomach cancer tissue, human tonsil tissue, mouse kidney tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD10 Ready-To-Use IHC Kit. CD10 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:25000-1:100000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60034-3-Ig targets Neprilysin/CD10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, pig
Immunogen Neprilysin/CD10 fusion protein Ag0427 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name membrane metallo-endopeptidase
Synonyms MME, MME,CD10, Enkephalinase, EC:3.4.24.11, CD10
Calculated Molecular Weight 750 aa, 86 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC101658
Gene Symbol CD10
Gene ID (NCBI) 4311
RRIDAB_10641991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08473
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).

实验方案

Product Specific Protocols
WB protocol for Neprilysin/CD10 antibody 60034-3-IgDownload protocol
IHC protocol for Neprilysin/CD10 antibody 60034-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols