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- KD/KO Validated
MMP1 Polyclonal antibody
MMP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
203
验证数据展示
经过测试的应用
Positive WB detected in | U-87 MG cells, NIH/3T3 cells |
Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells, HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10371-2-AP targets MMP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, rabbit, bovine |
Immunogen | MMP1 fusion protein Ag0547 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | matrix metallopeptidase 1 (interstitial collagenase) |
Synonyms | Interstitial collagenase, EC:3.4.24.7, CLGN, CLG, 27 kDa interstitial collagenase |
Calculated Molecular Weight | 54 kDa |
Observed Molecular Weight | 45-48 kDa;55-62 kDa |
GenBank Accession Number | BC013875 |
Gene Symbol | MMP1 |
Gene ID (NCBI) | 4312 |
RRID | AB_2297741 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P03956 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for MMP1 antibody 10371-2-AP | Download protocol |
IHC protocol for MMP1 antibody 10371-2-AP | Download protocol |
IF protocol for MMP1 antibody 10371-2-AP | Download protocol |
FC protocol for MMP1 antibody 10371-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Res Identification of TRA2B-DNAH5 fusion as a novel oncogenic driver in human lung squamous cell carcinoma. | ||
Biomaterials Material-driven immunomodulation and ECM remodeling reverse pulmonary fibrosis by local delivery of stem cell-laden microcapsules | ||
Biomaterials Macromechanics and polycaprolactone fiber organization drive macrophage polarization and regulate inflammatory activation of tendon in vitro and in vivo. | ||
Acta Biomater ROS-responsive magnesium-containing microspheres for antioxidative treatment of intervertebral disc degeneration | ||
Br J Cancer Chemerin suppresses hepatocellular carcinoma metastasis through CMKLR1-PTEN-Akt axis. | ||
Bioact Mater Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration. |