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MMP1 Polyclonal antibody

MMP1 Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

FC, IF, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10371-2-AP

Print datasheet

Synonyms

CLG, CLGN, Fibroblast collagenase, Interstitial collagenase, Matrix metalloproteinase 1, MMP 1, MMP1



经过测试的应用

Positive WB detected inU-87 MG cells, NIH/3T3 cells
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

产品信息

10371-2-AP targets MMP1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMP1 fusion protein Ag0547
Full Name matrix metallopeptidase 1 (interstitial collagenase)
Synonyms CLG, CLGN, Fibroblast collagenase, Interstitial collagenase, Matrix metalloproteinase 1, MMP 1, MMP1
Calculated molecular weight 54 kDa
Observed molecular weight 52-56 kDa, 62 kDa
GenBank accession numberBC013875
Gene symbol MMP1
UniprotP03956
Gene ID (NCBI) 4312
RRIDAB_2297741
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMP1, also named as CLG, belongs to the peptidase M10A family. MMP is the main enzyme that cleaves fibrillar collagen, namely types I, II, III, VII, and X.It is involved in cell migration and invasion, and is frequently up-regulated in cancer cells. It can be cleavage to two major forms (22 kDa and 27 kDa) by undergoing autolytic,and the minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.The sizes of pro-MMP-1 and active MMP-1 from human synovial fibroblasts have been reported as 52 to 56 kDa, depending on glycosylation, and 41 to 45 kDa, respectively.In addition ,a band of 62-kDa observed in the current study is pro-MMp-1(PMID:9418730).

实验方案

Product Specific Protocols
WB protocol for MMP1 antibody 10371-2-APDownload protocol
IF protocol for MMP1 antibody 10371-2-APDownload protocol
FC protocol for MMP1 antibody 10371-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Exp Ther Med

Expression variations and clinical significance of MMP-1, MMP-2 and inflammatory factors in serum of patients with deep venous thrombosis of lower extremity.

Authors - Tangshan Zhang
humanWB

Int J Oncol

25-HC decreases the sensitivity of human gastric cancer cells to 5-fluorouracil and promotes cells invasion via the TLR2/NF-κB signaling pathway.

Authors - Saisai Wang
humanWB

J Exp Clin Cancer Res

ZRANB2/SNHG20/FOXK1 Axis regulates Vasculogenic mimicry formation in glioma.

Authors - Xiaozhi Li
ratWB

Phytomedicine

Kadsura heteroclita stem suppresses the onset and progression of adjuvant-induced arthritis in rats.

Authors - Huanghe Yu
ratWB

Front Pharmacol

Schisandrin A Inhibits the IL-1β-Induced Inflammation and Cartilage Degradation via Suppression of MAPK and NF-κB Signal Pathways in Rat Chondrocytes.

Authors - Chang Tu
ratWB,IHC

Acta Biochim Biophys Sin (Shanghai)

PMID: 30883649

Authors - Tong Zhao