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验证数据展示

  • WB analysis using 30805-1-AP

    WB analysis using 30805-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30805-1-AP (MMP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 30805-1-AP

    IHC staining of human cervical cancer using 30805-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 30805-1-AP (MMP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MMP1 antibody included
  • IF Staining of HepG2 using 30805-1-AP

    IF Staining of HepG2 using 30805-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using MMP1 antibody (30805-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

MMP1 Polyclonal antibody

MMP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30805-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Interstitial collagenase, EC:3.4.24.7, CLGN, CLG, 27 kDa interstitial collagenase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inCaco-2 cells, HepG2 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MMP1 Ready-To-Use IHC Kit. MMP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30805-1-AP targets MMP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name matrix metallopeptidase 1 (interstitial collagenase)
Synonyms Interstitial collagenase, EC:3.4.24.7, CLGN, CLG, 27 kDa interstitial collagenase
Calculated Molecular Weight 54 kDa
Observed Molecular Weight22-27 kDa, 42-52 kDa
GenBank Accession NumberBC013875
Gene Symbol MMP1
Gene ID (NCBI) 4312
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP03956
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MMP1 antibody 30805-1-APDownload protocol
IHC protocol for MMP1 antibody 30805-1-APDownload protocol
IF protocol for MMP1 antibody 30805-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols