• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MMP11 Polyclonal antibody

MMP11 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30615-1-AP

Print datasheet

Synonyms

Matrix metalloproteinase 11, MMP 11, MMP11, SL 3, ST3, STMY3, Stromelysin 3

验证数据展示

  • WB analysis using 30615-1-AP

    WB analysis using 30615-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30615-1-AP (MMP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 30615-1-AP

    IF Staining of A549 using 30615-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MMP11 antibody (30615-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells, A549 cells, T-47D cells
Positive IF/ICC detected inA549 cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30615-1-AP targets MMP11 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen MMP11 fusion protein Ag31191 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name matrix metallopeptidase 11 (stromelysin 3)
Synonyms Matrix metalloproteinase 11, MMP 11, MMP11, SL 3, ST3, STMY3, Stromelysin 3
Calculated Molecular Weight 55 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberNM_005940
Gene Symbol MMP11
Gene ID (NCBI) 4320
RRIDAB_3086374
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24347
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Matrix metalloproteinase 11 (MMP11), also named stromelysin-3, is a member of the stromelysin subgroup belonging to MMPs superfamily. The role of MMP11 in cancer progression has been shown by several preclinical observations: its expression promotes tumor take in mice, homing of malignant epithelial cells, cancer progression by remodeling extracellular matrix, and antiapoptotic and antinecrotic effect on tumor cells (PMID: 19509157, 26892540). MMP11 has a calculated molecular mass of 55 kDa.

实验方案

Product Specific Protocols
WB protocol for MMP11 antibody 30615-1-APDownload protocol
IF protocol for MMP11 antibody 30615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols