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验证数据展示

  • WB analysis using 30615-1-AP

    WB analysis using 30615-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30615-1-AP (MMP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 30615-1-AP

    IF Staining of A549 using 30615-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MMP11 antibody (30615-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

MMP11 Polyclonal antibody

MMP11 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30615-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human and More (1)

应用

WB, IF/ICC, ELISA and More (1)

别名

Matrix metalloproteinase 11, MMP 11, MMP11, SL 3, ST3, STMY3, Stromelysin 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, A549 cells, T-47D cells
Positive IF/ICC detected inA549 cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

30615-1-AP targets MMP11 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用IF
经测试反应性 Human
文献引用反应性human
免疫原 MMP11 fusion protein Ag31191 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 matrix metallopeptidase 11 (stromelysin 3)
别名 Matrix metalloproteinase 11, MMP 11, MMP11, SL 3, ST3, STMY3, Stromelysin 3
计算分子量 55 kDa
观测分子量50-55 kDa
GenBank蛋白编号NM_005940
基因名称 MMP11
Gene ID (NCBI) 4320
RRIDAB_3086374
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP24347
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Matrix metalloproteinase 11 (MMP11), also named stromelysin-3, is a member of the stromelysin subgroup belonging to MMPs superfamily. The role of MMP11 in cancer progression has been shown by several preclinical observations: its expression promotes tumor take in mice, homing of malignant epithelial cells, cancer progression by remodeling extracellular matrix, and antiapoptotic and antinecrotic effect on tumor cells (PMID: 19509157, 26892540). MMP11 has a calculated molecular mass of 55 kDa.

实验方案

Product Specific Protocols
WB protocol for MMP11 antibody 30615-1-APDownload protocol
IF protocol for MMP11 antibody 30615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cancer Cell

Single-cell multi-stage spatial evolutional map of esophageal carcinogenesis

Authors - Jiang Chang
View Article