验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30102-1-AP targets MMP24 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | MMP24 fusion protein Ag31597 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | matrix metallopeptidase 24 (membrane-inserted) |
| 别名 | Matrix metalloproteinase 24, MMP 24, MMP24, MMP25, MT MMP 5, MT MMP5, MT5 MMP, MT5MMP, MTMMP5 |
| 计算分子量 | 73KD |
| 观测分子量 | 55-58 kDa |
| GenBank蛋白编号 | NM_006690 |
| 基因名称 | MMP24 |
| Gene ID (NCBI) | 10893 |
| RRID | AB_3086228 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y5R2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP24 (MT5-MMP) is a membrane type matrix metalloproteinase attached to the cell membrane by a transmembrane domain (PMID: 30654475). MMP24 is mainly expressed in the nervous system, hippocampus, and cerebellum (PMID: 10727639,35177031). MMP24 may negatively regulate the aggressiveness of cancer cells under the stiff ECM environment during tumor progression (PMID: 32093160). MMP24 can be detected as 62-65 kDa and 55-58 kDa (PMID: 16495457,20942921,10622708).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MMP24 antibody 30102-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |