MMP26 Polyclonal antibody

MMP26 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18087-1-AP

Print datasheet

Synonyms

Matrix metalloproteinase 26, matrix metallopeptidase 26, Matrilysin-2, Matrilysin 2, Endometase



经过测试的应用

Positive WB detected inSKOV-3 cells, HEK-293 cells, human placenta tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSKOV-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18087-1-AP targets MMP26 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen MMP26 fusion protein Ag12806 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name matrix metallopeptidase 26
Synonyms Matrix metalloproteinase 26, matrix metallopeptidase 26, Matrilysin-2, Matrilysin 2, Endometase
Calculated Molecular Weight 262 aa, 30 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC101541
Gene Symbol MMP26
Gene ID (NCBI) 56547
RRIDAB_2878495
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRE1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMP26(matrix metallopeptidase 26) may hydrolyze collagen type IV, fibronectin, fibrinogen, beta-casein, type I gelatin and alpha-1 proteinase inhibitor.MMP26 is expressed in placenta and uterus and is widely expressed in malignant tumors from different sources as well as in diverse tumor cell lines(PMID: 10987280).The proenzyme without the signal peptide has a calculated molecular mass of 29 kDa and the processed enzyme has a calculated molecular mass of 19 kDa(PMID:15574774).This antibody is specific to MMP26.

实验方案

Product Specific Protocols
WB protocol for MMP26 antibody 18087-1-APDownload protocol
IHC protocol for MMP26 antibody 18087-1-APDownload protocol
IF protocol for MMP26 antibody 18087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Adv

Succinate metabolism and membrane reorganization drives the endotheliopathy and coagulopathy of traumatic hemorrhage

Authors - Sarah Abdullah
humanIHC

J Huazhong Univ Sci Technolog Med Sci

MMP26: A potential biomarker for prostate cancer.

Authors - Teng Cheng