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  • Featured Product
  • KD/KO Validated

MMP3 Monoclonal antibody, PBS Only

MMP3 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F5A9

Cat no : 66338-1-PBS

Print datasheet

Synonyms

SL-1, MMP-3, MMP 3, Matrix metalloproteinase-3, EC:3.4.24.17

验证数据展示

  • IHC staining of human breast cancer using 66338-1-Ig (same clone as 66338-1-PBS)

    IHC staining of human breast cancer using 66338-1-Ig (same clone as 66338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MMP3 antibody included
  • IHC staining of human breast cancer using 66338-1-Ig (same clone as 66338-1-PBS)

    IHC staining of human breast cancer using 66338-1-Ig (same clone as 66338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MMP3 antibody included
  • WB analysis of HeLa using 66338-1-Ig (same clone as 66338-1-PBS)

    WB analysis of HeLa using 66338-1-Ig (same clone as 66338-1-PBS)

    WB result of MMP3 antibody (66338-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-MMP3 transfected HeLa cells. This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • WB analysis using 66338-1-Ig (same clone as 66338-1-PBS)

    WB analysis using 66338-1-Ig (same clone as 66338-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • IHC staining of rat colon using 66338-1-Ig (same clone as 66338-1-PBS)

    IHC staining of rat colon using 66338-1-Ig (same clone as 66338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MMP3 antibody included
Planning an IHC experiment? We recommend our IHCeasy MMP3 Ready-To-Use IHC Kit. MMP3 primary antibody included.

产品信息

66338-1-PBS targets MMP3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen MMP3 fusion protein Ag12359 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name matrix metallopeptidase 3 (stromelysin 1, progelatinase)
Synonyms SL-1, MMP-3, MMP 3, Matrix metalloproteinase-3, EC:3.4.24.17
Calculated Molecular Weight 477 aa, 54 kDa
Observed Molecular Weight 45-60 kDa
GenBank Accession NumberBC074869
Gene Symbol MMP3
Gene ID (NCBI) 4314
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08254
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Matrix metalloproteinases (MMPs) play a critically important role in extracellular matrix remodeling and have been implicated in a number of key normal and pathologic processes.These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 activate procollagenase via two pathways: slow direct activation and rapid activation in conjunction with tissue or plasma proteinases. The pro-MMP3 (60 kDa) and the active MMP3 (47 kDa) can be detected through western blot.