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验证数据展示

  • WB analysis of SKOV-3 using 67990-1-Ig

    WB analysis of SKOV-3 using 67990-1-Ig

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of human pancreas cancer using 67990-1-Ig

    IF Staining of human pancreas cancer using 67990-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using MMP7 antibody (67990-1-Ig, Clone: 2E6D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human stomach cancer using 67990-1-Ig

    IHC staining of human stomach cancer using 67990-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67990-1-Ig (MMP7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 67990-1-Ig

    IHC staining of human stomach cancer using 67990-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67990-1-Ig (MMP7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-3 using 67990-1-Ig

    IF Staining of PC-3 using 67990-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using MMP7 antibody (67990-1-Ig, Clone: 2E6D6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

MMP7 Monoclonal antibody

MMP7 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 67990-1-Ig

产品说明书

CloneNo. 2E6D6

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

Matrix metalloproteinase-7, Matrix metalloproteinase 7, Matrin, Matrilysin, EC:3.4.24.23

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSKOV-3 cells, HT-29 cells, COLO 320 cells, A549 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman pancreas cancer tissue
Positive IF/ICC detected inPC-3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67990-1-Ig targets MMP7 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 MMP7 fusion protein Ag0550 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 matrix metallopeptidase 7 (matrilysin, uterine)
别名 Matrix metalloproteinase-7, Matrix metalloproteinase 7, Matrin, Matrilysin, EC:3.4.24.23
计算分子量 29 kDa
观测分子量27-30 kDa
GenBank蛋白编号BC003635
基因名称 MMP7
Gene ID (NCBI) 4316
RRIDAB_2918739
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP09237
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MMP7 antibody 67990-1-IgDownload protocol
IHC protocol for MMP7 antibody 67990-1-IgDownload protocol
IF protocol for MMP7 antibody 67990-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols