验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82885-1-RR targets MMP8 in IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | IF/ICC, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | matrix metallopeptidase 8 (neutrophil collagenase) |
| 别名 | 230030G11, CLG1, EC:3.4.24.34, HNC, Matrix metalloproteinase 8 |
| 计算分子量 | 467 aa, 53 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC074988 |
| 基因名称 | MMP8 |
| Gene ID (NCBI) | 4317 |
| RRID | AB_3670603 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P22894 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MMP8(Matrix metalloproteinase-8) an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells(PMID:15367487). Purified human neutrophil MMP-8 with proeMMP-8 at 70-75 KDa and active enzyme at 65 KDa and amniochorion proenzyme and active enzyme at 65 and 50 KDa,respectively(PMID: 28772283, PMID:15042023).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MMP8 antibody 82885-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |