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MMS19 Polyclonal antibody
MMS19 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, human brain tissue, PC-3 cells |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy MMS19 Ready-To-Use IHC Kit. MMS19 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 17 publications below |
| CoIP | See 1 publications below |
产品信息
16015-1-AP targets MMS19 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | MMS19 fusion protein Ag8792 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | MMS19 nucleotide excision repair homolog (S. cerevisiae) |
| Synonyms | hMMS19, MET18, MET18 homolog, MMS19, MMS19 like protein, MMS19L |
| Calculated Molecular Weight | 1030 aa, 113 kDa |
| Observed Molecular Weight | 103-113 kDa |
| GenBank Accession Number | BC006575 |
| Gene Symbol | MMS19 |
| Gene ID (NCBI) | 64210 |
| RRID | AB_2145043 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96T76 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MMS19 plays a critical role in the biogenesis of multiple Fe-S proteins, which functions in genomic stability, RNA polymerase II function, and telomere length regulation. MMS19 has a alanine- and leucine-rich N terminus. It possible has a role in nucleotide excision repair(NER) and RNA polymerase II(POL II) transcription by interacting with ERCC2/XPB helicases, both subunits of NER-transcription factor TFIIH. Also MMS19 involves in the regulation of ER. It can function in chromosome segregation by acting as a part of the mitotic spindle-associated MMXD complex.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MMS19 antibody 16015-1-AP | Download protocol |
| IF protocol for MMS19 antibody 16015-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Cytosolic Iron-Sulfur Assembly Is Evolutionarily Tuned by a Cancer-Amplified Ubiquitin Ligase.
| ||
Cell Metab Human CIA2A-FAM96A and CIA2B-FAM96B Integrate Iron Homeostasis and Maturation of Different Subsets of Cytosolic-Nuclear Iron-Sulfur Proteins.
| ||
Nat Commun Structural basis for substrate recognition and chemical inhibition of oncogenic MAGE ubiquitin ligases. | ||
Cell Rep The CIA Targeting Complex Is Highly Regulated and Provides Two Distinct Binding Sites for Client Iron-Sulfur Proteins.
| ||
Mol Metab Cdkal1, a type 2 diabetes susceptibility gene, regulates mitochondrial function in adipose tissue. |