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  • Featured Product
  • KD/KO Validated

MMS19 Monoclonal antibody, PBS Only

MMS19 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H6D8

Cat no : 66049-1-PBS

Print datasheet

Synonyms

hMMS19, MET18, MET18 homolog, MMS19, MMS19 like protein, MMS19L

验证数据展示

  • IF Staining of HeLa using 66049-1-Ig (same clone as 66049-1-PBS)

    IF Staining of HeLa using 66049-1-Ig (same clone as 66049-1-PBS)

    Immunofluorescent analysis of fixed Hela cells using 66049-1-Ig (MMS19 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 66049-1-Ig (same clone as 66049-1-PBS)

    IHC staining of mouse testis using 66049-1-Ig (same clone as 66049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66049-1-Ig (MMS19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MMS19 antibody included
  • IHC staining of mouse testis using 66049-1-Ig (same clone as 66049-1-PBS)

    IHC staining of mouse testis using 66049-1-Ig (same clone as 66049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66049-1-Ig (MMS19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MMS19 antibody included
  • WB analysis of HeLa using 66049-1-Ig (same clone as 66049-1-PBS)

    WB analysis of HeLa using 66049-1-Ig (same clone as 66049-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 66049-1-Ig (same clone as 66049-1-PBS)

    IP experiment of mouse brain using 66049-1-Ig (same clone as 66049-1-PBS)

    IP result of anti-MMS19 (IP:66049-1-Ig, 4ug; Detection:66049-1-Ig 1:2000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MMS19 Ready-To-Use IHC Kit. MMS19 primary antibody included.

产品信息

66049-1-PBS targets MMS19 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MMS19 fusion protein Ag8960 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name MMS19 nucleotide excision repair homolog (S. cerevisiae)
Synonyms hMMS19, MET18, MET18 homolog, MMS19, MMS19 like protein, MMS19L
Calculated Molecular Weight 1030 aa, 113 kDa
Observed Molecular Weight 103 kDa, 38 kDa
GenBank Accession NumberBC006575
Gene Symbol MMS19
Gene ID (NCBI) 64210
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96T76
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MMS19 (MMS19 nucleotide excision repair homolog), also known as MET18, is a 1,030 amino acid nuclear protein containing seven HEAT repeats that belongs to the MET18/MMS19 family. Via its interactions with TFIIH p80 and TFIIH p89 helicases, MMS19 plays a role in nucleotide excision repair (NER) and RNA polymerase II (Pol II) transcription. MMS19 may also function as a transcriptional coactivator of estrogen receptor. While ubiquitously expressed, highest levels of MMS19 have been found in testis. At least five distinct MMS19 protein isoforms exist, which are produced by alternative splicing events.