验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, U2OS cells |
| Positive IHC detected in | human breast cancer tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24697-1-AP targets MN1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | MN1 fusion protein Ag20344 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | meningioma (disrupted in balanced translocation) 1 |
| 别名 | MGCR, MGCR1, MGCR1 PEN, MN1 |
| 计算分子量 | 1320 aa, 136 kDa |
| 观测分子量 | 136 kDa |
| GenBank蛋白编号 | BC156879 |
| 基因名称 | MN1 |
| Gene ID (NCBI) | 4330 |
| RRID | AB_2879678 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q10571 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MN1 antibody 24697-1-AP | Download protocol |
| IHC protocol for MN1 antibody 24697-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Astroblastomas exhibit radial glia stem cell lineages and differential expression of imprinted and X-inactivation escape genes. | ||
EBioMedicine METTL14-mediated epitranscriptome modification of MN1 mRNA promote tumorigenicity and all-trans-retinoic acid resistance in osteosarcoma.
| ||
Acta Neuropathol MN1 immunohistochemistry is a sensitive diagnostic biomarker for primitive CNS tumors with MN1 fusion |
