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  • Featured Product
  • KD/KO Validated

MN1 Polyclonal antibody

MN1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 24697-1-AP

Print datasheet

Synonyms

MGCR, MGCR1, MGCR1 PEN, MN1

验证数据展示

  • WB analysis using 24697-1-AP

    WB analysis using 24697-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24697-1-AP (MN1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 24697-1-AP

    IHC staining of human breast cancer using 24697-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24697-1-AP (MN1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 24697-1-AP

    IHC staining of human breast cancer using 24697-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24697-1-AP (MN1 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inMCF-7 cells, U2OS cells
Positive IHC detected inhuman breast cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24697-1-AP targets MN1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen MN1 fusion protein Ag20344 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name meningioma (disrupted in balanced translocation) 1
Synonyms MGCR, MGCR1, MGCR1 PEN, MN1
Calculated Molecular Weight 1320 aa, 136 kDa
Observed Molecular Weight136 kDa
GenBank Accession NumberBC156879
Gene Symbol MN1
Gene ID (NCBI) 4330
RRIDAB_2879678
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ10571
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MN1 antibody 24697-1-APDownload protocol
IHC protocol for MN1 antibody 24697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC,IF

Nat Commun

Astroblastomas exhibit radial glia stem cell lineages and differential expression of imprinted and X-inactivation escape genes.

Authors - Norman L Lehman
View Article
humanWB,IHC

EBioMedicine

METTL14-mediated epitranscriptome modification of MN1 mRNA promote tumorigenicity and all-trans-retinoic acid resistance in osteosarcoma.

Authors - Hong-Bo Li
  • KD Validated
View Article