MNT Recombinant antibody

MNT Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240550A10

Cat no : 83648-1-RR

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Synonyms

Max binding protein MNT, MAX binding protein, MAD6, Class D basic helix-loop-helix protein 3, bHLHd3



经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, HEK-293T cells, Jurkat cells, A431 cells, HT-29 cells, C6 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy MNT Ready-To-Use IHC Kit. MNT primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83648-1-RR targets MNT in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, rat
Immunogen MNT fusion protein Ag34529 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name MAX binding protein
Synonyms Max binding protein MNT, MAX binding protein, MAD6, Class D basic helix-loop-helix protein 3, bHLHd3
Calculated Molecular Weight 582 aa, 62 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC117563
Gene Symbol MNT
Gene ID (NCBI) 4335
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99583
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mnt (Max binding protein), also known as MAD6, ROX, bHLHd3 (class D basic helix-loop-helix protein 3) or MXD6, is a 582 amino acid nuclear protein that forms a complex with Max (Myc-associated factor X) to repress transcription. Mnt contains one basic helix-loop-helix (bHLH) domain and is encoded by a gene that maps to human chromosome 17p13.3. Chromosome 17 comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.

实验方案

Product Specific Protocols
WB protocol for MNT antibody 83648-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols