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验证数据展示

  • WB analysis of K-562 using 15886-1-AP

    WB analysis of K-562 using 15886-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15886-1-AP (MOBKL3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 15886-1-AP

    IHC staining of human colon cancer using 15886-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15886-1-AP (MOBKL3 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 15886-1-AP

    IHC staining of human colon cancer using 15886-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15886-1-AP (MOBKL3 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of K-562 using 15886-1-AP

    IP experiment of K-562 using 15886-1-AP

    IP result of anti-MOBKL3 (IP:15886-1-AP, 4ug; Detection:15886-1-AP 1:300) with K-562 cells lysate 4000ug.

MOBKL3 Polyclonal antibody

MOBKL3 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 15886-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

PHOCN, Mps one binder kinase activator-like 3, MOB4, CGI-95, 2C4D

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15886-1-AP targets MOBKL3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MOBKL3 fusion protein Ag8671 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MOB1, Mps One Binder kinase activator-like 3 (yeast)
别名 PHOCN, Mps one binder kinase activator-like 3, MOB4, CGI-95, 2C4D
计算分子量 225 aa, 26 kDa
观测分子量 24 kDa
GenBank蛋白编号BC005237
基因名称 MOBKL3
Gene ID (NCBI) 25843
RRIDAB_10858478
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3A3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MOBKL3 antibody 15886-1-APDownload protocol
IHC protocol for MOBKL3 antibody 15886-1-APDownload protocol
IP protocol for MOBKL3 antibody 15886-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols