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MOBKL3 Polyclonal antibody

MOBKL3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15886-1-AP

Print datasheet

Synonyms

PHOCN, Mps one binder kinase activator-like 3, MOB4, CGI-95, 2C4D

验证数据展示

  • WB analysis of K-562 using 15886-1-AP

    WB analysis of K-562 using 15886-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15886-1-AP (MOBKL3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 15886-1-AP

    IHC staining of human colon cancer using 15886-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15886-1-AP (MOBKL3 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 15886-1-AP

    IHC staining of human colon cancer using 15886-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15886-1-AP (MOBKL3 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of K-562 using 15886-1-AP

    IP experiment of K-562 using 15886-1-AP

    IP result of anti-MOBKL3 (IP:15886-1-AP, 4ug; Detection:15886-1-AP 1:300) with K-562 cells lysate 4000ug.

经过测试的应用

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15886-1-AP targets MOBKL3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MOBKL3 fusion protein Ag8671 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name MOB1, Mps One Binder kinase activator-like 3 (yeast)
Synonyms PHOCN, Mps one binder kinase activator-like 3, MOB4, CGI-95, 2C4D
Calculated Molecular Weight 225 aa, 26 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC005237
Gene Symbol MOBKL3
Gene ID (NCBI) 25843
RRIDAB_10858478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3A3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MOBKL3 antibody 15886-1-APDownload protocol
IHC protocol for MOBKL3 antibody 15886-1-APDownload protocol
IP protocol for MOBKL3 antibody 15886-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols