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MOBP Polyclonal antibody

MOBP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12472-1-AP

Print datasheet

Synonyms

MOBP

验证数据展示

  • IHC staining of human gliomas using 12472-1-AP

    IHC staining of human gliomas using 12472-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12472-1-AP (MOBP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 12472-1-AP

    IHC staining of human gliomas using 12472-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12472-1-AP (MOBP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse brain using 12472-1-AP

    WB analysis of mouse brain using 12472-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12472-1-AP (MOBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:300-1:600
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12472-1-AP targets MOBP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen MOBP fusion protein Ag3156 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myelin-associated oligodendrocyte basic protein
Synonyms MOBP
Calculated Molecular Weight 183 aa, 21 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC022471
Gene Symbol MOBP
Gene ID (NCBI) 4336
RRIDAB_10644161
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13875
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MOBP antibody 12472-1-APDownload protocol
IHC protocol for MOBP antibody 12472-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Neurobiol Dis

Inhibition of CXCR2 enhances CNS remyelination via modulating PDE10A/cAMP signaling pathway

Authors - Cheng Ju
View Article
mouseWB

Glia

ST8SIA2 promotes oligodendrocyte differentiation and the integrity of myelin and axons.

Authors - Lukasz Mateusz Szewczyk
View Article
humanIHC

Bioengineered

Four specific biomarkers associated with the progression of glioblastoma multiforme in older adults identified using weighted gene co-expression network analysis

Authors - Yushi Yang
View Article