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验证数据展示

  • WB analysis of HepG2 using 16395-1-AP

    WB analysis of HepG2 using 16395-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16395-1-AP (MOCOS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16395-1-AP

    IHC staining of human liver using 16395-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16395-1-AP (MOCOS antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 16395-1-AP

    IHC staining of human liver using 16395-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16395-1-AP (MOCOS antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of HepG2 using 16395-1-AP

    IP experiment of HepG2 using 16395-1-AP

    IP result of anti-MOCOS (IP:16395-1-AP, 4ug; Detection:16395-1-AP 1:300) with HepG2 cells lysate 4000ug.

  • IF Staining of HepG2 using 16395-1-AP

    IF Staining of HepG2 using 16395-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MOCOS antibody (16395-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

MOCOS Polyclonal antibody

MOCOS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16395-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Molybdenum cofactor sulfurtransferase, Molybdenum cofactor sulfurase, MoCo sulfurase, MCS, hMCS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16395-1-AP targets MOCOS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MOCOS fusion protein Ag9487 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 molybdenum cofactor sulfurase
别名 Molybdenum cofactor sulfurtransferase, Molybdenum cofactor sulfurase, MoCo sulfurase, MCS, hMCS
计算分子量 888 aa, 98 kDa
观测分子量 98 kDa
GenBank蛋白编号BC012079
基因名称 MOCOS
Gene ID (NCBI) 55034
RRIDAB_2297842
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96EN8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MOCOS(Molybdenum cofactor sulfurase) is also named as MCS, MOS, hMCS and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. It catalyzes the removal of elemental sulfur from cysteine to produce alanine. Sulfation of molybdenum is essential for xanthine dehydrogenase (XDH) and aldehyde oxidase (ADO) enzymes in which molybdenum cofactor is liganded by 1 oxygen and 1 sulfur atom in active form. Defects in MOCOS are the cause of xanthinuria type 2 (XU2)(PMID:17368066).

实验方案

Product Specific Protocols
WB protocol for MOCOS antibody 16395-1-APDownload protocol
IHC protocol for MOCOS antibody 16395-1-APDownload protocol
IF protocol for MOCOS antibody 16395-1-APDownload protocol
IP protocol for MOCOS antibody 16395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols