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验证数据展示

  • WB analysis using 68308-1-Ig

    WB analysis using 68308-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68308-1-Ig (MOCS2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IF Staining of HeLa using 68308-1-Ig

    IF Staining of HeLa using 68308-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MOCS2 antibody (68308-1-Ig, Clone: 3H2D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

MOCS2 Monoclonal antibody

MOCS2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68308-1-Ig

产品说明书

CloneNo. 3H2D5

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

MCBPE, MOCO1, MOCO1 A, MOCS2, MOCS2A, MOCS2B, MPTS, Sulfur carrier protein MOCS2A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68308-1-Ig targets MOCS2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 MOCS2 fusion protein Ag6282 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 molybdenum cofactor synthesis 2
别名 MCBPE, MOCO1, MOCO1 A, MOCS2, MOCS2A, MOCS2B, MPTS, Sulfur carrier protein MOCS2A
计算分子量 21 kDa
观测分子量21 kDa
GenBank蛋白编号BC046097
基因名称 MOCS2
Gene ID (NCBI) 4338
RRIDAB_2935387
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO96033
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MOCS2(Molybdenum cofactor synthesis 2) is a heterotetrameric synthase comprised of 2 small (MOCS2A) and 2 large (MOCS2B) subunits. MOCS2 acts as a sulfur carrier required for molybdopterin biosynthesis. The two MOCS2 proteins A and B are subunits of molybdopterin synthase incorporating sulfur groups delivered by the MOCS3 sulfurylase.

实验方案

Product Specific Protocols
WB protocol for MOCS2 antibody 68308-1-IgDownload protocol
IF protocol for MOCS2 antibody 68308-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols