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验证数据展示

  • WB analysis using 68308-1-Ig

    WB analysis using 68308-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68308-1-Ig (MOCS2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IF Staining of HeLa using 68308-1-Ig

    IF Staining of HeLa using 68308-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MOCS2 antibody (68308-1-Ig, Clone: 3H2D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

MOCS2 Monoclonal antibody

MOCS2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68308-1-Ig

Print datasheet

CloneNo. 3H2D5

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

MCBPE, MOCO1, MOCO1 A, MOCS2, MOCS2A, MOCS2B, MPTS, Sulfur carrier protein MOCS2A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68308-1-Ig targets MOCS2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen MOCS2 fusion protein Ag6282 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name molybdenum cofactor synthesis 2
Synonyms MCBPE, MOCO1, MOCO1 A, MOCS2, MOCS2A, MOCS2B, MPTS, Sulfur carrier protein MOCS2A
Calculated Molecular Weight 21 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC046097
Gene Symbol MOCS2
Gene ID (NCBI) 4338
RRIDAB_2935387
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO96033
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MOCS2(Molybdenum cofactor synthesis 2) is a heterotetrameric synthase comprised of 2 small (MOCS2A) and 2 large (MOCS2B) subunits. MOCS2 acts as a sulfur carrier required for molybdopterin biosynthesis. The two MOCS2 proteins A and B are subunits of molybdopterin synthase incorporating sulfur groups delivered by the MOCS3 sulfurylase.

实验方案

Product Specific Protocols
WB protocol for MOCS2 antibody 68308-1-IgDownload protocol
IF protocol for MOCS2 antibody 68308-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols