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验证数据展示

  • WB analysis using 68370-1-Ig

    WB analysis using 68370-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68370-1-Ig (MORC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • WB analysis using 68370-1-Ig

    WB analysis using 68370-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68370-1-Ig (MORC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

MORC3 Monoclonal antibody

MORC3 Monoclonal Antibody for WB, ELISA
Cat No. 68370-1-Ig

产品说明书

CloneNo. 1E1E1

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse

应用

WB, ELISA

别名

KIAA0136, MORC3, NXP2, ZCW5, ZCWCC3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, NIH/3T3 cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, 4T1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68370-1-Ig targets MORC3 in WB, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse
免疫原 MORC3 fusion protein Ag21867 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 MORC family CW-type zinc finger 3
别名 KIAA0136, MORC3, NXP2, ZCW5, ZCWCC3
计算分子量 939 aa, 107 kDa
观测分子量130 kDa
GenBank蛋白编号BC132731
基因名称 MORC3
Gene ID (NCBI) 23515
RRIDAB_3085090
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ14149
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MORC3, also named as MORC family CW-type zinc finger protein 3 or NXP2, is a 939 amino acid protein, which contains 1 CW-type zinc finger. MORC3 localizes in the nucleus and are expressed in heart, placenta, skeletal muscle, brain, pancreas, lung, liver, but not kidney. MORC3 as Nuclear factor which forms MORC3-NBs (nuclear bodies) via an ATP-dependent mechanism. Sumoylated MORC3-NBs can also associate with PML-NBs and recruits TP53 and SP100 to PML-NBs, thus regulating TP53 activity. It may be required for influenza A transcription during viral infection. The calculated molecular weight of MORC3 is a 107 kDa, but the Sumoylated MORC3 protein is about 130 kDa.

实验方案

Product Specific Protocols
WB protocol for MORC3 antibody 68370-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols