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MORF4L1 Polyclonal antibody

MORF4L1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55257-1-AP

Print datasheet

Synonyms

Eaf3, FWP006, HSPC008, HSPC061, HsT17725, MORF related gene 15 protein, MORF4L1, MORFRG15, mortality factor 4 like 1, MRG15, PP368, Protein MSL3 1, S863 6

验证数据展示

  • WB analysis of HEK-293 using 55257-1-AP

    WB analysis of HEK-293 using 55257-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 55257-1-AP

    IHC staining of human skin using 55257-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:200 (under 40x lens).

  • IP experiment of HEK-293 using 55257-1-AP

    IP experiment of HEK-293 using 55257-1-AP

    IP result of anti-MORF4L1 (IP:55257-1-AP, 4ug; Detection:55257-1-AP 1:800) with HEK-293 cells lysate 1200ug.

经过测试的应用

Positive WB detected inHEK-293 cells, A549 cells, mouse brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman skin tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55257-1-AP targets MORF4L1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mortality factor 4 like 1
Synonyms Eaf3, FWP006, HSPC008, HSPC061, HsT17725, MORF related gene 15 protein, MORF4L1, MORFRG15, mortality factor 4 like 1, MRG15, PP368, Protein MSL3 1, S863 6
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 35-37 kDa
GenBank Accession NumberNM_006791
Gene Symbol MORF4L1
Gene ID (NCBI) 10933
RRIDAB_10836938
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBU8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MORF4L1, also named as MRG15, belongs to the MRG family. It is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. It is also component of the mSin3A complex which acts to repress transcription by deacetylation of nucleosomal histones. (PMID:14966270) This antibody has no cross-reaction to MORF4L2.

实验方案

Product Specific Protocols
WB protocol for MORF4L1 antibody 55257-1-APDownload protocol
IHC protocol for MORF4L1 antibody 55257-1-APDownload protocol
IP protocol for MORF4L1 antibody 55257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols