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MPG Polyclonal antibody, PBS Only

MPG Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11481-2-PBS

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Synonyms

验证数据展示

  • IP experiment of HepG2 using 11481-2-AP (same clone as 11481-2-PBS)

    IP experiment of HepG2 using 11481-2-AP (same clone as 11481-2-PBS)

    IP result of anti-MPG (IP:11481-2-AP, 4ug; Detection:11481-2-AP 1:300) with HepG2 cells lysate 2200 ug. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 11481-2-AP (same clone as 11481-2-PBS)

    WB analysis of HepG2 using 11481-2-AP (same clone as 11481-2-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 11481-2-AP (same clone as 11481-2-PBS)

    IHC staining of human prostate cancer using 11481-2-AP (same clone as 11481-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11481-2-AP (MPG antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

产品信息

11481-2-PBS targets MPG in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MPG fusion protein Ag2034 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name N-methylpurine-DNA glycosylase
Synonyms
Calculated Molecular Weight 293 aa, 32 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC014991
Gene Symbol MPG
Gene ID (NCBI) 4350
RRIDAB_2282144
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29372
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

N-methylpurine DNA glycosylase (MPG), an initiator glycosylase of the base excision repair (BER) pathway, typically repairs alkyl-induced DNA adducts, many of which are associated with genomic instability and tumorigenic risk [PMID:22801474] MPG initiates BER by removing TMZ-induced N3-methyladenine and N7-methylguanine base lesions, leaving abasic sites (AP sites) in DNA for further processing by BER [PMID:21377995]. MPG involves in modulating the genotoxic threshold response induced by the Sn2 alkylating agent ethyl methanesulfonate (EMS) [PMID:21071726].