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验证数据展示

  • WB analysis using 68417-1-Ig

    WB analysis using 68417-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68417-1-Ig (MPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HEK-293 using 68417-1-Ig

    WB analysis of HEK-293 using 68417-1-Ig

    WB result of MPI antibody (68417-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MPI transfected HEK-293 cells.

  • IP experiment of Jurkat using 68417-1-Ig

    IP experiment of Jurkat using 68417-1-Ig

    IP result of anti-MPI (IP:68417-1-Ig, 4ug; Detection:68417-1-Ig 1:2000) with Jurkat cells lysate 2280 ug.

  • IHC staining of mouse brain using 68417-1-Ig

    IHC staining of mouse brain using 68417-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68417-1-Ig (MPI antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 68417-1-Ig

    IHC staining of mouse brain using 68417-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68417-1-Ig (MPI antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NIH/3T3 using 68417-1-Ig

    IF Staining of NIH/3T3 using 68417-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using MPI antibody (68417-1-Ig, Clone: 3B10A6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO验证

MPI Monoclonal antibody

MPI Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 68417-1-Ig

产品说明书

CloneNo. 3B10A6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Phosphomannose isomerase, Phosphohexomutase, Mannose-6-phosphate isomerase, mannose phosphate isomerase, Mannose 6 phosphate isomerase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293 cells, rat brain tissue, A549 cells, LnCaP cells, Jurkat cells, K-562 cells, pig brain tissue
Positive IP detected inJurkat cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68417-1-Ig targets MPI in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 MPI fusion protein Ag5475 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mannose phosphate isomerase
别名 Phosphomannose isomerase, Phosphohexomutase, Mannose-6-phosphate isomerase, mannose phosphate isomerase, Mannose 6 phosphate isomerase
计算分子量 47 kDa
观测分子量 40-47 kDa
GenBank蛋白编号BC046357
基因名称 MPI
Gene ID (NCBI) 4351
RRIDAB_3085132
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP34949
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MPI(mannose-6-phosphate isomerase) is also named as PMI1 and belongs to the mannose-6-phosphate isomerase type 1 family. It is a zinc metalloenzyme that catalyzes the reversible isomerization of mannose-6-phosphate and fructose-6-phosphate. MPI plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. This proterin has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for MPI antibody 68417-1-IgDownload protocol
IHC protocol for MPI antibody 68417-1-IgDownload protocol
IF protocol for MPI antibody 68417-1-IgDownload protocol
IP protocol for MPI antibody 68417-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols