验证数据展示
经过测试的应用
| Positive WB detected in | DU 145 cells, human brain tissue, Y79 cells, HeLa cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17710-1-AP targets MPP5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | MPP5 fusion protein Ag12010 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) |
| 别名 | Protein PALS1, Protein associated with Lin-7 1, PALS1, Membrane protein, palmitoylated 5, MAGUK p55 subfamily member 5 |
| 计算分子量 | 675 aa, 77 kDa |
| 观测分子量 | 70-80 kDa |
| GenBank蛋白编号 | BC095485 |
| 基因名称 | MPP5 |
| Gene ID (NCBI) | 64398 |
| RRID | AB_2282012 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N3R9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MPP5, also named as PALS1, belongs to the MAGUK family which interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. MPP5 is thought to organize intracellular PALS1-CRB-MUPP1 protein scaffolds in the retina that are involved in maintenance of photoreceptor-Mu¨ller glia cell adhesion. MPP5 plays a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MPP5 antibody 17710-1-AP | Download protocol |
| IHC protocol for MPP5 antibody 17710-1-AP | Download protocol |
| IF protocol for MPP5 antibody 17710-1-AP | Download protocol |
| IP protocol for MPP5 antibody 17710-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuron Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate. | ||
Neuron Mosaic Analysis with Double Markers Reveals Distinct Sequential Functions of Lgl1 in Neural Stem Cells. | ||
Dev Cell Multicellular rosettes link mesenchymal-epithelial transition to radial intercalation in the mouse axial mesoderm | ||
Cell Death Differ The evolutionary history of the polyQ tract in huntingtin sheds light on its functional pro-neural activities. | ||
J Am Soc Nephrol Crumbs2 Is an Essential Slit Diaphragm Protein of the Renal Filtration Barrier. |
