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MPP6 Polyclonal antibody

MPP6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11575-1-AP

Print datasheet

Synonyms

MAGUK p55 subfamily member 6, MPP6, p55T, PALS2, VAM 1, VAM1, Veli associated MAGUK 1

验证数据展示

  • WB analysis using 11575-1-AP

    WB analysis using 11575-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11575-1-AP (MPP6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 11575-1-AP

    IHC staining of mouse testis using 11575-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11575-1-AP (MPP6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 11575-1-AP

    IHC staining of mouse testis using 11575-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11575-1-AP (MPP6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, SMMC-7721 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11575-1-AP targets MPP6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen MPP6 fusion protein Ag2158 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6)
Synonyms MAGUK p55 subfamily member 6, MPP6, p55T, PALS2, VAM 1, VAM1, Veli associated MAGUK 1
Calculated Molecular Weight 540 aa, 61 kDa
Observed Molecular Weight 55-61 kDa
GenBank Accession NumberBC023638
Gene Symbol MPP6
Gene ID (NCBI) 51678
RRIDAB_2250848
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZW5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MPP6 antibody 11575-1-APDownload protocol
IHC protocol for MPP6 antibody 11575-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Front Immunol

Elevated MPP6 expression correlates with an unfavorable prognosis, angiogenesis and immune evasion in hepatocellular carcinoma

Authors - Qianqian Cheng
View Article