验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, A2780 cells, SKOV-3 cells |
| Positive IP detected in | SKOV-3 cells |
| Positive IHC detected in | mouse ovary tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-Fro detected in | mouse brain tissue |
| Positive IF/ICC detected in | Hela cells |
Planning an IHC experiment? We recommend our IHCeasy MPRIP Ready-To-Use IHC Kit. MPRIP primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 1 publications below |
产品信息
20040-1-AP targets MPRIP in WB, IHC, IF/ICC, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-Fro, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myosin phosphatase Rho interacting protein |
| 别名 | M RIP, MRIP, M-RIP, Myosin phosphatase Rho-interacting protein, p116Rip |
| 计算分子量 | 117 kDa |
| 观测分子量 | 116-140 kDa |
| GenBank蛋白编号 | NM_015134 |
| 基因名称 | MPRIP |
| Gene ID (NCBI) | 23164 |
| RRID | AB_10694433 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6WCQ1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MPRIP, also named as KIAA0864, MRIP, RHOIP3 and p116Rip, is required for the regulation of the actin cytoskeleton by RhoA and ROCK1. MRPIP targets myosin phosphatase to the actin cytoskeleton. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells. The antibody recognizes the C-term of isoform2 and isoform3 of MPRIP.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MPRIP antibody 20040-1-AP | Download protocol |
| IHC protocol for MPRIP antibody 20040-1-AP | Download protocol |
| IF protocol for MPRIP antibody 20040-1-AP | Download protocol |
| IP protocol for MPRIP antibody 20040-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Glia Ermin is a p116RIP -interacting protein promoting oligodendroglial differentiation and myelin maintenance. | ||
RNA DAP5 drives translation of specific mRNA targets with upstream ORFs in human embryonic stem cells | ||
J Cancer High Expression of lncRNA AFAP1-AS1 Promotes the Progression of Colon Cancer and Predicts Poor Prognosis. |
