验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
60103-1-Ig targets MPV17 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 免疫原 | MPV17 fusion protein Ag0312 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | MpV17 mitochondrial inner membrane protein |
| 别名 | |
| 计算分子量 | 20 kDa |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | BC001115 |
| 基因名称 | MPV17 |
| Gene ID (NCBI) | 4358 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Caprylic Acid/Ammonium Sulfate Precipitation |
| UNIPROT ID | P39210 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MPV17, also termed as SYM1, encodes an inner mitochondrial membrane protein. It’s potential candidate that may account for the mitochondrial (mt) DNA depletion syndromes (MDDS) characterized by a severe, tissue-specific decrease of mtDNA copy number and ultimate organ failure. It’s a human ortholog of the mouse kidney disease gene Mpv17. Absence or malfunction of MPV17 causes oxidative phosphorylation (OXPHOS) failure and mtDNA depletion, not only in affected individuals but also in Mpv17-/- mice. MPV17 contribute to ROS homeostasis, but the downstream signaling is unknown yet. Catalog#60103-1-Ig is a mouse monoclonal antibody raised against the full-length of human MPV17.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MPV17 antibody 60103-1-Ig | Download protocol |
| IHC protocol for MPV17 antibody 60103-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |