验证数据展示
经过测试的应用
Positive IHC detected in | mouse small intestine tissue, rat dorsal root ganglion tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse small intestine tissue |
推荐稀释比
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
23640-1-AP targets MRGPRD in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IHC, IF-P, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | MRGPRD fusion protein Ag20316 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | MAS-related GPR, member D |
Synonyms | MRGD, Mas-related G-protein coupled receptor member D, MAS related GPR, member D, G-protein coupled receptor TGR7, Beta-alanine receptor |
Calculated Molecular Weight | 321 aa, 36 kDa |
GenBank Accession Number | BC156660 |
Gene Symbol | MRGPRD |
Gene ID (NCBI) | 116512 |
RRID | AB_3669414 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen Affinity purified |
UNIPROT ID | Q8TDS7 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MRGPRD (MAS-related G-protein coupled receptor, D), also referred to as hGPCR45 or TGR7, was identified as a novel GPCR in murine and human genomes. As for MRGD, its expression was found in dorsal root ganglia (DRG) and co-localized with Vanilloid receptor-1 (VR-1), which is an essential receptor for heat and pain sensation (PMID: 22715397).
实验方案
Product Specific Protocols | |
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IHC protocol for MRGPRD antibody 23640-1-AP | Download protocol |
IF protocol for MRGPRD antibody 23640-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |