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MRP4 Monoclonal antibody

MRP4 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B10B4

Cat No : 67230-1-Ig

Print datasheet

Synonyms

ABCC4, MOAT B, MOATB, MRP4

验证数据展示

  • WB analysis using 67230-1-Ig

    WB analysis using 67230-1-Ig

    Non-boil and boiled HepG2, A549, and PC-12 cell lysates were subjected to SDS PAGE followed by western blot with 67230-1-Ig (ABCC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate hyperplasia using 67230-1-Ig

    IHC staining of human prostate hyperplasia using 67230-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 67230-1-Ig (ABCC4 antibody) at dilution of 1:1000 (under 40x lens)..

  • FC experiment of A549 using 67230-1-Ig

    FC experiment of A549 using 67230-1-Ig

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human MRP4 (67230-1-Ig, Clone:1B10B4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

67230-1-Ig targets MRP4 in WB, FC, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, IHC, WB, ELISA
Cited ApplicationsIHC
Tested Reactivity Human
Cited Reactivityhuman
Immunogen MRP4 fusion protein Ag10171 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATP-binding cassette, sub-family C (CFTR/MRP), member 4
Synonyms ABCC4, MOAT B, MOATB, MRP4
Calculated Molecular Weight 859 aa, 97 kDa
Observed Molecular Weight 160-180 kDa
GenBank Accession NumberBC041560
Gene Symbol ABCC4
Gene ID (NCBI) 10257
RRIDAB_2882517
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO15439
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MRP4 (also known as ABCC4), belonging to ATP-binding cassette (ABC) transporter family, is a multi-transmembrane protein that is able to transport a range of organic anionic compounds (both endogenous and xenobiotic) out of the cell. It can exclude various drugs thus is considered as potential target to prevent drug-resistance.

实验方案

Product Specific Protocols
WB protocol for MRP4 antibody 67230-1-IgDownload protocol
IHC protocol for MRP4 antibody 67230-1-IgDownload protocol
FC protocol for MRP4 antibody 67230-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Genet

Clinical Significance and Potential Mechanisms of ATP Binding Cassette Subfamily C Genes in Hepatocellular Carcinoma.

Authors - Xin Zhou
View Article