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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 29792-1-AP

    WB analysis using 29792-1-AP

    Boiled and unboiled MCF-7 lysates were subjected to SDS PAGE followed by western blot with 29792-1-AP (ABCC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 29792-1-AP

    IHC staining of human ovary cancer using 29792-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29792-1-AP (ABCC5 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ABCC5 Polyclonal antibody

ABCC5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29792-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

MRP5, MOATC, MOAT C, EC:7.6.2.-, ATP-binding cassette sub-family C member 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, unboiled HepG2 cells, unboiled MCF-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29792-1-AP targets ABCC5 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 ABCC5 fusion protein Ag31680 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP-binding cassette, sub-family C (CFTR/MRP), member 5
别名 MRP5, MOATC, MOAT C, EC:7.6.2.-, ATP-binding cassette sub-family C member 5
计算分子量 161 kDa
观测分子量161-200 kDa
GenBank蛋白编号NM_005688
基因名称 ABCC5
Gene ID (NCBI) 10057
RRIDAB_3086162
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15440
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCC5, also named as MOAT-C, pABC11, SMRP, and MRP5, belongs to the ABC transporter superfamily, ABCC family, and Conjugate transporter (TC 3.A.1.208) subfamily. ABCC5 acts as a multispecific organic anion pump that can transport nucleotide analogs. ABCC5 functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that ABCC5 provides resistance to thiopurine anticancer drugs, 6-mercaptopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl) adenine. ABCC5 may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients. Alternative splicing of this gene has been detected; however, the complete sequence and translation initiation site are unclear. Since it is glycosylated, the apparent molecular weight of ABCC5 could be variable, ranging from 161 kDa to 200 kDa (PMID: 10893247; PMID: 15897250; PMID: 31338999).

实验方案

Product Specific Protocols
WB protocol for ABCC5 antibody 29792-1-APDownload protocol
IHC protocol for ABCC5 antibody 29792-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols