验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, HeLa cells, mouse lung tissue, Jurkat cells, COS-7 cells, rat lung tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
| IHC | See 1 publications below |
产品信息
16241-1-AP targets MRPL13 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat, monkey |
| 文献引用反应性 | human |
| 免疫原 | MRPL13 fusion protein Ag9105 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mitochondrial ribosomal protein L13 |
| 别名 | L13, L13A, L13mt, MRP L13, MRPL13, RPL13, RPML13 |
| 计算分子量 | 178 aa, 21 kDa |
| 观测分子量 | 20-23 kDa |
| GenBank蛋白编号 | BC009190 |
| 基因名称 | MRPL13 |
| Gene ID (NCBI) | 28998 |
| RRID | AB_2145705 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BYD1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MRPL13, also named as 39S ribosomal protein L13, mitochondrial or L13mt, is a 178 amino acid protein, which belongs to the ribosomal protein L13P family. MRPL13 localizes in the mitochondrion and interacts with OXA1L. MRPL13 is involved in mitochondrial proteins biosynthesis, ribosomal structure and biogenesis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MRPL13 antibody 16241-1-AP | Download protocol |
| IHC protocol for MRPL13 antibody 16241-1-AP | Download protocol |
| IP protocol for MRPL13 antibody 16241-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Transcription-independent role for human mitochondrial RNA polymerase in mitochondrial ribosome biogenesis. | ||
Genomics Proteomics Bioinformatics Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses. | ||
J Cell Sci CLUH couples mitochondrial distribution to the energetic and metabolic status. | ||
Life (Basel) Molecular Investigation of Mitochondrial RNA19 Role in the Pathogenesis of MELAS Disease |
